A primary area of research in the Knoll laboratory centers on studying the host/pathogen interactions of the intracelluar parasite Toxoplasma gondii. Toxoplasma causes abnormal fetal neurological development and encephalitis in immunocompromised patients. Toxoplasma is a member of the coccidian family of parasites that include Plasmodium (causative agent of malaria) and Cryptosporidium (causative agent of water-borne outbreaks of diarrhea). We are using a library signature-tagged mutants and a chronic infection mouse model to isolate genes important for virulence and developmental regulation.
Payne AJ, Neal LM, Knoll LJ.
2013. Fusidic acid is an effective treatment against Toxoplasma gondii and Listeria monocytogenes in vitro, but not in mice. Parasitol. Res. 112(11):3859-63
Moser LA, Pollard AM, Knoll LJ.
2013. A genome-wide siRNA screen to identify host factors necessary for growth of the parasite Toxoplasma gondii. PLoS ONE 8(6):e68129 (PMC3695992)
Tobin CM, Knoll LJ.
2012. A patatin-like protein protects Toxoplasma gondii from degradation in a nitric oxide-dependent manner. Infect. Immun. 80(1):55-61 (PMC3255658)
Payne TM, Payne AJ, Knoll LJ.
2011. A Toxoplasma gondii mutant highlights the importance of translational regulation in the apicoplast during animal infection. Mol. Microbiol. 82(5):1204-16
Payne TM, Lund PJ, Knoll LJ.
2011. A transmembrane domain containing pellicle protein of Toxoplasma gondii enhances virulence and invasion after extracellular stress. Mol. Biochem. Parasitol. 179(2):107-10 (PMC3156857)
O'Brien KB, Schultz-Cherry S, Knoll LJ.
2011. Parasite-mediated upregulation of NK cell-derived gamma interferon protects against severe highly pathogenic H5N1 influenza virus infection. J. Virol. 85(17):8680-8 (PMC3165849)
Rooney PJ, Neal LM, Knoll LJ.
2011. Involvement of a Toxoplasma gondii chromatin remodeling complex ortholog in developmental regulation. PLoS ONE 6(5):e19570 (PMC3104990)
Craver MP, Rooney PJ, Knoll LJ.
2010. Isolation of Toxoplasma gondii development mutants identifies a potential proteophosphogylcan that enhances cyst wall formation. Mol. Biochem. Parasitol. 169(2):120-3 (PMC2791180)
Pollard AM, Knoll LJ, Mordue DG.
2009. The role of specific Toxoplasma gondii molecules in manipulation of innate immunity. Trends Parasitol. 25(11):491-4 (PMC2771455)
Pollard AM, Skariah S, Mordue DG, Knoll LJ.
2009. A transmembrane domain-containing surface protein from Toxoplasma gondii augments replication in activated immune cells and establishment of a chronic infection. Infect. Immun. 77(9):3731-9 (PMC2737990)
Frankel MB, Knoll LJ.
2009. The ins and outs of nuclear trafficking: unusual aspects in apicomplexan parasites. DNA Cell Biol. 28(6):277-84 (PMC2903460)
Frankel MB, Knoll LJ.
2008. Functional analysis of key nuclear trafficking components reveals an atypical Ran network required for parasite pathogenesis. Mol. Microbiol. 70(2):410-20 (PMC2577059)
Van TT, Kim SK, Camps M, Boothroyd JC, Knoll LJ.
2007. The BSR4 protein is up-regulated in Toxoplasma gondii bradyzoites, however the dominant surface antigen recognised by the P36 monoclonal antibody is SRS9. Int. J. Parasitol. 37(8-9):877-85
Frankel MB, Mordue DG, Knoll LJ.
2007. Discovery of parasite virulence genes reveals a unique regulator of chromosome condensation 1 ortholog critical for efficient nuclear trafficking. Proc. Natl. Acad. Sci. U.S.A. 104(24):10181-6 (PMC1891257)
Craver MP, Knoll LJ.
2007. Increased efficiency of homologous recombination in Toxoplasma gondii dense granule protein 3 demonstrates that GRA3 is not necessary in cell culture but does contribute to virulence. Mol. Biochem. Parasitol. 153(2):149-57