Charles Czuprynski Home

Personnel at the Food Research Institute, UW–Madison

	Charles Czuprynski
	Director, Food Research Institute Professor, Pathobiological Sciences, School of Veterinary Medicine mailing address: 1311 Microbial Sciences, 1550 Linden Drive, University of Wisconsin–Madison Madison, WI 53706–1521
	telephone:608-263-6826 email

Research Focus	Education	Selected Publications	return to:
Current Research Projects	Program Affiliations	Outreach / Recent Presentations	FRI home page \| FRI Personnel

RESEARCH INTERESTS
Pathogenesis of listeriosis and salmonellosis; Bovine respiratory disease; Bone marrow toxicity of polycyclic aromatic hydrocarbons; Johne’s disease; Food safety research; Training of veterinarians

RESEARCH FOCUS		EDUCATION
The focus of our laboratory is on the pathogenesis and host response to foodborne bacterial pathogens. We have a particular interest in how growth conditions affect the virulence of Listeria monocytogenes and Salmonella enterica, and how dietary and environmental constituents might after the immune response to these bacterial pathogens. Our lab’s interests include both pre-harvest and post-harvest food safety issues. Examples of the former include our research on bovine respiratory disease, a major economic problem for beef and dairy cattle, and a reason for much of the antibiotic use in cattle. We also place an emphasis on providing veterinarians, and veterinary students, with research training related to food safety.		BA 1976, Knox College; Biology PhD 1980, University of Wisconsin–Madison; Medical Microbiology Postdoc 1983, National Jewish Hospital; Immunology

CURRENT RESEARCH PROJECTS		PROGRAM AFFILIATIONS
Effect of growth conditions on pathogenesis of salmonellosis and listeriosis. Role of serotype 4b genes in pathogenesis of listeriosis. Does exposure to vinegar salad dressing affect survival of pathogenic bacteria on leafy greens? Viral/bacterial synergism in bovine respiratory disease. Arylhydrocarbon receptor in resistance to listeriosis. Effects of route and metabolism of polycyclic aromatic hydrocarbons on bone marrow toxicity. Walter Renk Endowed Laboratory in Food Safety.		University of Wisconsin–Madison Molecular and Environmental Toxicology Center IAFP, ASM (former Division E chair), SOT, AAI, AAVI (former President), WVMA, USDA Regional Committee on Bovine Respiratory Disease

SELECTED PUBLICATIONS Search Pubmed for Publications Authored by Czuprynski CJ

Berryman, L., and C. J. Czuprynski. 2008. Flavonoid treatment of murine macrophages does not alter ingestion or intracellular survival of Listeria monocytogenes. Immunopharmacol. Immunotoxicol. 30:217–226.
Kuckleburg, C., R. Tiwari, and C. J. Czuprynski. 2008. Endothelial cell apoptosis induced by bacteria-activated platelets requires caspase-8 and -9 and generation of reactive oxygen species. Thrombosis Haemostasis 99(2):363–372.
McClenahan, D., K. Hellenbrand, D. Atapattu, N. Aulik, D. Carlton, A. Kapur, and C. J. Czuprynski. 2008. Effects of lipopolysaccharide and Mannheimia haemolytica leukotoxin on bovine lung microvascular endothelial cells and alveolar epithelial cells. Clin. Vaccine Immunol. 15(2):338–347.
Neudeck, B., N. Faith, and C. Czuprynski. 2008. The poloxamer P85 is protective against Listeria monocytogenes invasion. Foodborne Pathogen. Dis. 5:859–865.
Atapattu, D., and C. J. Czuprynski. 2007. Mannheimia haemolytica leukotoxin binds to lipid rafts in bovine lymphoblastoid cells (BL-3) and is internalized in a dynamin-2 and clathrin-dependent manner. Infect. Immun. 75:4719–4725.
Behling-Kelly, E., D. McClenahan, K. S. Kim, and C. J. Czuprynski. 2007. Viable Haemophilus somnus induce myosin light chain kinase-dependent decrease in brain endothelial cell monolayer resistance. Infect. Immun. 75:4572–4581.
Behling-Kelly, E., and C. J. Czuprynski. 2007. Endothelial cells as active participants in veterinary infections and inflammatory disorders. Anim. Health Res. Rev. 8:47–58.
Faith, N. G., S. Kathariou, B. L. Neudeck, J. B. Luchansky, and C. J. Czuprynski. 2007. A P60 mutant of Listeria monocytogenes is impaired in its ability to cause infection in intragastrically inoculated mice. Microb. Pathog. 42:237–241.
Kuckleburg, C. J., S. F. Elswaifi, T. J. Inzana, and C. J. Czuprynski. 2007. Expression of phosphorylcholine by Histophilus somni induces bovine platelet aggregation. Infect. Immun. 75:1045–1049.
Peterson, L. D., N. G. Faith, and C. J. Czuprynski. 2007. Resistance of Listeria monocytogenes strain F2365 to synthetic gastric fluid is greater following growth on ready-to-eat deli turkey meat than in brain heart infusion broth. J. Food Prot. 70:2589–2595.
Shi, L., N. G. Faith, and C. J. Czuprynski. 2007. The aryl hydrocarbon receptor is required for optimal innate immunity to Listeria monocytogenes infection in mice. J. Immunol. 179:6952–6962.
Woo, S. R., J. A. Heintz, R. Albrecht, R. G. Barletta, and C. J. Czuprynski. 2007. Life and death in bovine monocytes: the fate of Mycobacterium avium subsp. paratuberculosis. Microb. Pathogen. 43:106–113.
Woo, S. R., R. G. Barletta, and C. J. Czuprynski. 2007. Addition of ATP is cytotoxic to mononuclear phagocytes but does not induce killing of intracellular Mycobacterium paratuberculosis. Clin. Vaccine Immunol. 14:1078–1083.
Behling-Kelly, E., H. Vonderheid, K. S. Kim, L. B. Corbeil, and C. J. Czuprynski. 2006. Roles of cellular activation and sulfated glycans in Haemophilus somnus adherence to bovine brain microvascular endothelial cells. Infect. Immun. 74:5311–5318.
Galvan, N., C. J. Czuprynski, and C. Jefcoate. 2006. Benzo(a)pyrene and 7,12-dimethylbez(a)anthracene differentially affect bone marrow cells of the lymphoid and myeloid lineages. Toxicol. Appl. Pharmacol. 213:105–116.
McClenahan, D., R. Kreuger, M. Kehrli, C. Thomas, and C. J. Czuprynski. 2006. In situ hybridization analysis of interleukin-8 expression in bovine mammary gland tissue sections from calves with experimental Escherichia coli mastitis. Comp. Immunol. Microbiol. Infect. Dis. 29:127–137.
Sylte, M. J., C. J. Kuckleburg, F. P. Leite, T. J. Inzana, and C. J. Czuprynski. 2006. Tumor necrosis factor-alpha enhances Haemophilus somnus lipooligosaccharide-induced apoptosis of bovine endothelial cells. Vet. Immunol. Immunopathol. 110:303–309.
Woo, S. R., J. Sotos, A. P. Hart, R. G. Barletta, and C.J. Czuprynski. 2006. Bovine monocytes and a macrophage cell line differ in their ability to phagocytose and support the intracellular survival of Mycobacterium avium subsp. paratuberculosis. Vet. Immunol. Immunopathol. 110:109–120.

OUTREACH / SELECTED RECENT PRESENTATIONS

“Pathogenesis and Innate Immunity of Listeria monocytogenes Infection.” University of Georgia, April 20, 2009.
“Phenotypic characterization of gtcA transposon mutants of serotype 4b Listeria monocytogenes” (poster). IAFP 2008 (International Association for Food Protection annual meeting), August 3–6, 2008, Columbus, Ohio.
“The role of adherence-associated surface antigens in foodborne listeriosis.” National Restaurant Association Quality Assurance Executive Study Group, October 16, 2007, Rogers, AR.
“The aryl hydrocarbon receptor: a new player in innate immunity to bacterial infection.” UW–Madison Immunology Symposium, May 29, 2008, Madison, WI.
“The role of gtcA in the pathogenesis of gastrointestinal listeriosis” at ISOPOL XVI, The 16th International Symposium on Problems of Listeriosis, Savannah, GA, March 21, 2007.
Organizer and Chair of a mini-symposium on “Role of Surface Antigens in the Pathogenicity of Listeriosis” at ISOPOL XVI, The 16th International Symposium on Problems of Listeriosis, Savannah, GA, March 23, 2007.
“Environmental, host and bacterial factors that influence the pathogenesis of gastrointestinal listeriosis,” NAFS-ARS Mini-Symposium, IAFP Meeting, Aug. 9, 2003, New Orleans.
“Environmental, host and bacterial factors that influence the virulence of Listeria monocytogenes.” 91st Annual Meeting of the International Association for Food Protection, Aug. 9, 2004, Phoenix, AZ.
“Mechanisms of immunosuppression in foodborne disease.” 91st Annual Meeting of the International Association for Food Protection, Aug. 10, 2004, Phoenix, AZ.
“Role of tumor necrosis factor and protein kinase R in the bone marrow cell response to polycyclic aromatic hydrocarbons.” Univ. New Mexico College of Pharmacy, Sept. 27, 2004, Albuquerque, NM.
“The unlikely development of a veterinary immunologist.” Distinguished Veterinary Immunologist Award Lecture, CRWAD Meeting, Nov. 14, 2004, Chicago, IL.
“Does growth of Listeria monocytogenes on sliced turkey affect its virulence and ability to invade intestinal epithelial cells?” USDA Food Safety 32.0 Principal Investigator’s Meeting, Oct. 19, 2005, River Falls, WI
“New insights into the pathogenesis of gastrointestinal listeriosis,” Food Research Institute Seminar, Feb. 22, 2006, Madison WI
“The role of gtcA in the pathogenesis of gastrointestinal listeriosis.” ISOPOL XVI, The 16th International Symposium on Problems of Listeriosis, March 21, 2007, Savannah, GA.
“The role of adherence-associated surface antigens in foodborne listeriosis.” National Restaurant Association Quality Assurance Executive Study Group. October 16, 2007, Rogers, AR.
“The aryl hydrocarbon receptor: a new player in innate immunity to bacterial infection.” UW-Madison Immunology Symposium, May 29, 2008, Madison, WI.

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